microplate reader (Thermo Fisher)

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Thermo Fisher microplate reader
Microplate Reader, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bovine+serum/Bovine+Serum+Albumin/pmc13320252-61-8-12
Average 99 stars, based on 1 article reviews

microplate reader - by Bioz Stars, 2026-09

99/100 stars

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Cell Culture:

Article Title: Extracellular adenosine released by mesenchymal stromal cells from young hosts boosts the antimicrobial activity of neutrophils from aged hosts against Streptococcus pneumoniae .
Article Snippet: .. Cells were cultured in tissue culture–treated plates in a medium containing minimum essential medium α (α-MEM) with Glutamin (Gibco) and supplemented with 10% heat-inactivated fetal bovine serum (Gibco) and 1% penicillin/streptomycin antibiotics (Thermo Fisher Scientific) for 3 days at 37°C in 5% CO2 and normal atmospheric oxygen to allow adherence of MSCs. ..

Article Title: M1 macrophage-conditioned medium and Vitex agnus-castus effects on bone tissue organization in ovariectomized rats: In vivo and in vitro study.
Article Snippet: Background: Bone repair is a highly coordinated process regulated by the local microenvironment, in which macrophage polarization plays a key role, particularly under estrogen-deficient conditions.. Natural immunomodulatory compounds, such as Vitex agnus-castus (monk’s pepper; VAC), may influence macrophage-mediated responses and exert osteoprotective effects.. This study investigated how M1 macrophage-conditioned medium (CM) and VAC, alone or in combination, influence bone tissue organization and osteogenic activity in ovariec-

Article Title: Design, synthesis, and pharmacological evaluation of thiazolo[5,4-d]pyrimidines hybridized with lipoic acid or catechol. Novel A 2A adenosine receptor antagonists in neuroinflammatory diseases.
Article Snippet: The effect of synthesized compounds was investigated on murine microglial BV-2 cell line (Elabscience Biotechnology Inc. Houston, TX, USA, Cat. No. EP-CL-0493). .. Cells were cultured in RPMI medium (Sigma Aldrich, Germany) with 10% heat-inactivated fetal bovine serum (Gibco, USA) and 1% penicillin/streptomycin (Gibco, USA) at 37 ◦C in a humidified incubator with 5% CO2. .. Upon confluence, cells were collected with 0.25% trypsin (Sigma Aldrich, Germany) and 0.02% EDTA (Sigma Aldrich, Germany) in phosphate saline buffer (PBS), centrifuged (300×g for 5 min), counted and seeded in different cell culture dishes, depending on the experiment.

Modification:

Article Title: Antioxidant and anti-inflammatory effects of the extract from rhizomes of Belamcanda chinensis (L.) DC. on human keratinocyte HaCaT cells
Article Snippet: The cell line identity was verified by matching the supplier-provided STR profile with the HaCaT reference profile from Ubigene ( https://www.ubigene.us/ ). .. The cells were grown in Dulbecco's modified Eagle's medium (Gibco; Thermo Fisher Scientific, Inc.) with 10% heat-inactivated fetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) with 100 U/ml penicillin and 100 g/ml streptomycin. ..

Mutagenesis:

Article Title: Hypomorphic biliverdin reductase a mutations define bilirubin anti-malarial threshold
Article Snippet: .. Primary mouse BMDMs from wild-type (WT) and indicated mutant mice ( Blvra −/− , Blvra G17A , Blvra E97A ) were grown for 6 days in RPMI (GIBCO) supplemented with 10% heat-inactivated fetal bovine serum (GIBCO), 10% L929 conditioned media and 1% penicillin and streptomycin., BMDMs were seeded at a density of 2 × 10 6 cells/well in 6 well plates, 1 × 10 6 cells/well in 12 well plates or 100 × 10 4 cells/well in 8-chamber slides (μ-slide, Ibidi) and allowed to adhere overnight. .. Cells were stimulated with 50 μM heme (Frontier Scientific) for 24h, 500 nM insulin (Merck) for 10 min or 10 μM MG-132 (Merck) for 6h and treated for downstream analysis.

Mouse Assay:

Saline:

Article Title: Mpox Seroprevalence among Sex Workers and Men who have Sex with Men Preceding Sustained Urban Outbreak in the Democratic Republic of the Congo
Article Snippet: In brief, each peptide was first conjugated to bovine serum albumin before covalent 154 attachment to magnetic beads with distinct fluorescent signatures (Luminex Corp, Austin, TX, 155 USA). .. A master-mix bead solution containing Phosphate Buffered Saline (PBS) with 156 0.75 mol/NaCl, 5% heat-inactivated fetal bovine serum (Gibco-Invitrogen, France), 1% bovine 157 serum albumin, and 0.2% Tween-20 (Sigma-Aldrich, France) was added to each well of 96-well 158 flat-bottom chimney plates (Greiner Bio One, Germany). .. Diluted serum samples (1:200) were 159 subsequently added to individual wells in duplicate; following washing steps, a secondary biotin-160 labeled anti-anti-human IgG antibody (BD-Pharmingen, France) was added.

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